These cookies ensure basic functionalities and security features of the website, anonymously. Necessary cookies are absolutely essential for the website to function properly. MC20-2 or MC1020-2 Growth Medium used for incubating the spores strips which have not been sterilized (positive control) should turn to yellow for the results to be valid. the sterilization process was effective). If after 24 hours (Steam) or 48 hours (Form) no color change is visible on MC20-2 or MC1020-2 growth medium, the result is negative (i.e. It is advisable to make observations every 10 hours.Ĭolor change of MC20-2 or MC1020-2 indicating medium to yellow means a failure of the sterilization process. Incubate processed spore strips along with the strip used as a positive control between 55-62✬ during a maximum of 24 hours for Steam or 48 hours for Formaldehyde. Positive control guarantees that the incubation was carried out under appropriate conditions.Ħ.
Both, processed spore strip and the strip used as positive control must belong to the same batch. Use a spore strip which has not been subjected to the sterilization process as a positive control each time a processed strip is incubated. IMPORTANT: Use latex gloves and a mask when transferring spore strips from the envelope to growth medium tube. WARNING: Avoid spore strip contact with any kind of surface including the outer part of Growth Medium tube and of Bionova® BT50 envelope. laminar flow cabinet) and transfer the spore strip with a sterile clamp to MC20-2 or MC1020-2 Growth Medium tube or any other appropriate growth medium. Tear up the envelope at one end very carefully under sterility conditions (eg. After sterilization process has finished, remove Bionova® BT50 envelope from the package for processing and incubation.ĥ. A typical problematic area is the load center.Ĥ. Place the envelope in those areas you a priori consider more inaccessible for sterilizing agent. Pack it in an appropriate package along with material to be sterilized, according to recommended sterilization practices. Identify Bionova® BT50 envelope by writing on its back the sterilizer number (in case of having more than one), load number and processing date.Ģ. A final readout should therefore be performed after 24 hours of growth medium incubation between 55-62✬ for Steam sterilization processes control, or 48 hours between 55-62✬ for Formaldehyde sterilization processes control.ġ. If the sterilization process was effective, MC20-2 or MC1020-2 Indicating Medium will remain its original color. If the sterilization process was not effective, MC20-2 or MC1020-2 Growth Medium will turn to yellow after incubation between 55-62✬, thus indicating the existence of living Geobacillus stearothermophilus spores on the strips. Spore strips should be incubated in Bionova® MC20-2 or MC1020-2 Growth Medium tube or in appropiate Growth medium for Geobacillus stearothermophilus ATCC 7953 growth. The envelope containing Bionova® BT50 Spore Strip is specifically designed to control Steam 121 ✬-135 ✬ (Steam) and Formaldehyde (Form) sterilization processes. Geobacillus stearothermophilus (ATCC 7953) 10^6 spores per strip. BT50/6 / Spore Strips Biological Indicator for Steam and Formaldehyde sterilization processes.
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